Benthic communities vary according to changes in the environmental factors in addition to human impacts. The present study aimed to investigate the changes of macro-benthos community in three sites at the Red Sea coast of Egypt during summer (2019) and winter (2020). Benthic communities were studied at 5 sampling groups (HS: El-Hamrawen summer, MS: Sedy Malek summer, HW: El-Hamrawen winter, MW: Sedy Malek winter, PW Porto Ghalb winter). The results indicated that dissolved oxygen is the most important factor in macro-benthos composition, followed by carbonate content, salinity, water temperature, and conductivity. The structure of macro-benthos community spatially and seasonally differs according to changes of environmental factors. Related to changes of environmental variations benthos recorded the highest abundance and diversity for (Pw) community and the lowest one for (Hs). Seasonal changes in temperature may be the main factors influence other environmental factors which affect benthos community. This led to conclude that global warming could have significant consequences for vital coastal fauna.