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221414

Genetic Diversity and Phylogenetic Relationship among Three Apocrita Species (Hymenoptera) From Qena Governorate, Egypt Based On RAPD Markers

Article

Last updated: 28 Dec 2024

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Subjects

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Tags

Zoology and Entomology

Abstract

The genomic DNA of three Apocrita species (Hymenoptera) namely, Vespa orientalis [Vespoidea; Vespidae], Xylocopa aestuans [Apoidea; Apidae] and Apis mellifera [Apoidea; Apidae] has been subjected to RAPD-PCR analysis with 10 deca-oligonucleotides [A-01, A-02, A-03, A-04, A-05, A-07, A-08, A-09, A-11, and A-12]. All primers produced discrete and instructive RAPD bands of various lengths ranging from 200 to 3000 base pair. Ninety seven amplified fragments/bands were produced, from them 4 bands were common (monomorphic) and 93 were polymorphic; those correspond to a level of polymorphism of 95.88 % with an average number of polymorphic fragments per primer of 9.3 band. The Xylocopa aesteanus recorded the highest band frequencies among the studied species. Based on the Nei-72 distance matrix of genetic and the unweighted pair group method average (UPGMA), the three species under study are related to each other and have common ancestor. However, both Vespa orientalis (Vespidae) and Apis mellifera (Apidae) are genetically closer. The splitting up Xylocopa austeanus into sister clade to Vespa orientalis and Apis mellifera may be a sign to reconsider placement of genus Xylocopa within Apidae.

DOI

10.21608/aunj.2017.221414

Keywords

Genetic diversity, Phylogeny, Apocrita, Vespa orientalis, Xylocopa aestuanus, Apis mellifera, RAPD-PCR, Qena, Clade

Volume

46

Article Issue

1

Related Issue

31576

Issue Date

2017-06-01

Receive Date

2022-02-23

Publish Date

2017-06-01

Page Start

13

Page End

25

Print ISSN

2812-5029

Online ISSN

2812-5037

Article File

AUNJ_Volume 46_Issue 1_Pages 13-25.pdf

PDF . 503.5kB

Link

https://aunj.journals.ekb.eg/article_221414.html

Detail API

https://aunj.journals.ekb.eg/service?article_code=221414

Order

221,414

Type

Novel Research Articles

Type Code

2,242

Publication Type

Journal

Publication Title

Assiut University Journal of Multidisciplinary Scientific Research

Publication Link

https://aunj.journals.ekb.eg/

MainTitle

Genetic Diversity and Phylogenetic Relationship among Three Apocrita Species (Hymenoptera) From Qena Governorate, Egypt Based On RAPD Markers

Details

Type

Article

Created At

23 Jan 2023