Primary screening of the antibacterial activity of yeast methanolic extracts against six pathogenic bacterial strains were examined by disc diffusion methods. Antimicrobial activities of the highest 10 molecular identified yeast strains methanolic extracts were confirmed against pathogenic (bacteria, yeast, dermatophyte and filamentous fungi) by using disc diffusion method and wells diffusion method were tested. The major metabolites in methanolic extract of the highest antimicrobial active Rhodotorula mucilaginosa AUMC13565 strain were determined by GC/MS analysis. The GC/MS analysis showed 41 active metabolites are classified based on their structure into 13 alcohols, 7 aldehyds, 6 fatty acids, 5 azoles derivatives and 10 other metabolites. All the available information about the detected metabolites were reported and included GC/MS %, Retention Time, CID number, molecular formula, molecular weight g/mol, uses and bioactivity. The most detected metabolites had many bioactivities included seven metabolites had anticancer activity followed by 5 from “anti-bacteria, antifungal and flavoring agents", 3 anti-inflammatory, 2 antioxidant, one metabolite from each “antidiuretic, estrogen receptor, insecticide, herbicide, plant growth regulator, antineoplastic diseases, antischizophrenia, immune system disorders nematotoxic, antimalaria, anti-leukemia and immunosuppressant agents according to the references in Pub Chem. citation.
Keywords: Yeast antimicrobial bioactivity; Rhodotorula mucilaginosa, GC/MS analysis.