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220936

CRITICAL PARAMETERS INFLUENCING HYPERTHERMIA-INDUCED CELL DEATH IN MYELOGENOUS LEUKEMIA CELL LINE (M-NFS-60)

Article

Last updated: 28 Dec 2024

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Subjects

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Tags

Zoology and Entomology

Abstract

Hyperthermia is sufficient to induce cell death in many cancer cells. Here we investigated the effect of some parameters that modulate hyperthermia-induced cell death in myelogenous leukemia cell line (M-NFS-60). Trypan blue exclusion method was used to measure M-NSF-60 cell viability after heat exposure to different temperatures (39, 41 and 43 ºC) for different periods of time (15, 30 and 60 min). Induced apoptotic cell death after heat exposure was investigated by western blot analysis for the expression level of active caspase-3 protein. The results of this work revealed that M-NFS-60 cells respond to hyperthermia in a graded fashion depend on heat load, time exposure and cell density. Heat exposure at 39 ºC and 41 ºC for 15, 30 and 60 min and at 43 ºC for 15 min caused cell growth arrest, however, at 43 ºC for 30 and 60 min it gradually inhibited the growth of M-NFS-60 cells. These data suggest that heat stress at 43 ºC at least for 30 min and more has therapeutic efficiency in leukemic cells. Finally, we determined heat load, time exposure and cell density as critical parameters influenced cytotoxic effect of hyperthermia on leukemic cells.

DOI

10.21608/aunj.2019.220936

Keywords

hyperthermia, heat load, time exposure, Cell density, myelogenous leukemia, M-NFS-60

Volume

48

Article Issue

1

Related Issue

31480

Issue Date

2019-06-01

Receive Date

2022-02-21

Publish Date

2019-06-01

Page Start

1

Page End

14

Print ISSN

2812-5029

Online ISSN

2812-5037

Article File

AUNJ_Volume 48_Issue 1_Pages 1-14.pdf

PDF . 808.3kB

Link

https://aunj.journals.ekb.eg/article_220936.html

Detail API

https://aunj.journals.ekb.eg/service?article_code=220936

Order

220,936

Type

Novel Research Articles

Type Code

2,242

Publication Type

Journal

Publication Title

Assiut University Journal of Multidisciplinary Scientific Research

Publication Link

https://aunj.journals.ekb.eg/

MainTitle

CRITICAL PARAMETERS INFLUENCING HYPERTHERMIA-INDUCED CELL DEATH IN MYELOGENOUS LEUKEMIA CELL LINE (M-NFS-60)

Details

Type

Article

Created At

23 Jan 2023