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220867

OPTIMIZATION OF THE CELLULASE ENZYME PRODUCTION BY BREVIBACTERIUM HALOTOLERANS ISOLATED FROM WADI EL-NATRUN, EGYPT

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Last updated: 05 Jan 2025

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Tags

Botany and Microbiology

Abstract

The present work was carried out to isolate the cellulose-decomposing bacteria (CDB) from Wadi El-Natrun, Egypt. Totally 40 isolates were isolated from saline mud samples. Eight out of 40 isolates exhibited high hydrolysis capacity of the Congo-Red screening method. The highly producer isolate (CDB2) was selected to optimize some nutritional and environmental factors for improving cellulase production. The bacterial isolate was identified on the basis of molecular characteristics using 16srRNA. The sequence analysis showed 99% similarity to Brevibacterium halotolerans, which is then deposited in the GenBank in NCBI under the accession number MN148622. The bacterium showed highly cellulase production by using carboxymethyl cellulose (CMC) as a sole carbon source. The results showed that the optimum conditions for cellulase production by the selected isolate were noticed when grown on CMC (20 g/L) at pH 8.0 incubated at 35οC in batch conditions with shaking. The results revealed that, the bacterial isolate showed potential cellulase production at stationary phase of growth corresponding to an incubation period of 3-5 days. The cellulase enzyme has high specificity to CMC considered to be an endo-1,4-gluca

DOI

10.21608/aunj.2020.220867

Keywords

Cellulose-decomposing bacteria, Cellulase, Brevibacterium halotolerans

Volume

49

Article Issue

1

Related Issue

31477

Issue Date

2020-06-01

Receive Date

2022-02-21

Publish Date

2020-06-01

Page Start

14

Page End

33

Print ISSN

2812-5029

Online ISSN

2812-5037

Article File

AUNJ_Volume 49_Issue 1_Pages 14-33.pdf

PDF . 630.0kB

Link

https://aunj.journals.ekb.eg/article_220867.html

Detail API

https://aunj.journals.ekb.eg/service?article_code=220867

Order

220,867

Type

Novel Research Articles

Type Code

2,242

Publication Type

Journal

Publication Title

Assiut University Journal of Multidisciplinary Scientific Research

Publication Link

https://aunj.journals.ekb.eg/

MainTitle

OPTIMIZATION OF THE CELLULASE ENZYME PRODUCTION BY BREVIBACTERIUM HALOTOLERANS ISOLATED FROM WADI EL-NATRUN, EGYPT

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Type

Article

Created At

23 Jan 2023