From July 2019 to June 2020, the prevalence of Cysticercus bovis was conducted to determine the percent of infection and, morphological, histological, and molecular characterizations of C.bovis on variant tissues. Results showed that the overall infection rate of C.bovis in cattle was (0.47 %) but in buffaloes was (0.04 %). Seasonal prevalence showed that the highest infection rate in cattle was in the spring season (0.56 %) and the lowest infection rate was in autumn (0.41 %), but in buffaloes, the infection rate was (0.05 %) in the summer season and (0.14 %) in the spring season. Organ distribution of C.bovis showed that in cattle the highest infection rate (64.43 %) was in heart muscle and the lowest infection rate (35.57 %) was in masseter muscles. In buffaloes, all positive cases were in cardiac muscles. PCR analysis using a primer of 18S rDNA gene produced size of 1600bp band at gel electrophoresis. Histopathological studies showed a cyst cavity formed by the larval stage beside necrosis and atrophy in different examined tissues.