Objectives: The incidence of Pseudomonas aeruginosa (P. aeruginosa), an important etiological cause of nosocomial infections, strains that produce beta-lactamases has been frequently recognized. Therefore, accurate and timely detection of isolates that produce carbapenemase is crucial. Aim of the work: The purpose of this study was to determine the frequency of the bla-IMP gene in carbapenem-resistant P. aeruginosa strains recovered from hospitals affiliated with Minia University. Patients and Methods: From June 2018 to September 2018, 40 P. aeruginosa strains isolated from various specimens were used in this work. Carbapenem resistant strains were gathered and analyzed for bla-IMP gene expression using PCR. Results: Out of 40 P. aeruginosa strains, 26 isolates were carbapenem resistant detected by (Vitek-2, bioMérieux, France) expressing the bla-IMP gene was in 46% of isolates. Conclusion: Carbapenem resistance in our hospitals have developed into a serious issue that calls for accurate diagnosis and efficient treatment. In this study, colistin was the antibiotic that worked the best against isolates that were resistant to carbapenem. To determine the primary source of resistance, it is advised to conduct genetic analyses for the most prevalent genes responsible for carbapenem resistance on a significant number of clinical specimens. However, given its expensive price, this might be restricted.