Background: Implant-supported overdentures are a use full treatment modality for edentulous patients. the implants retain dentures was a big improvement for the patients and offered new treatment alternatives. The aim was to evaluate and compare the effect of two different implant positions on strain developed around four implants supporting a maxillary overdenture with milled bar attachment. Methods:  Construction of A (3D) maxillary acrylic model was done from a cone beam computed tomography of an edentulous patient. Four implants were inserted bilaterally into an acrylic resin model in canines and premolar regions. Experimental overdentures with metal housings were constructed for each group to be connected to the implants in canine area with milled bar attachment (group I) or connected to the implants in premolar area with milled bar attachment.  Four linear strain gauges were bonded to the acrylic resin at the buccal and palatal surfaces of each implant. Strains were measured by using strain gauges by the aid universal testing machine to apply load at unilateral (canine and premolar side) and bilaterally. the data was collected and analyzed. Result: The result shown that strain was highest record at canine than premolar with either unilateral, or bilaterally loading. Conclusion: Within this study limitations, stresses are higher at the inserted implant at the canine area than those inserted in premolar area in milled bar attachment cases.