Background: Thalassemia is a hematologic disease caused by mutations in the genes coding for hemoglobin chains. The most common causes of genetic disorders in humans are mutations within the β-globin gene, of which 350 β-thalassemia mutations have been identified to date. The study aimed to assess hematological parameters in beta thalassemia major children in pediatric department of hematology, El-Minia university children and maternity hospital. Methods: This cross-sectional study was carried out from January 2021 to November 2021.Thirty beta thalassemic major children were taken during their regular follow up in the pediatric hematology outpatient clinic and hematology internal department, El-Minia University Children and Maternity Hospital the patients were divided into 2 groups; children underwent splenectomy and children without splenectomy. Blood samples were collected and analyzed for hematological parameters. The two groups: were subjected to careful detailed history taking, complete clinical examination, laboratory investigations including:  complete blood count (CBC), serum ferritin. Results: There was a statistically significant difference as regard platelet count between the two groups (P value 0.047). splenectomized children showed a higher platelet count than the other group.  There was statistically significantly increase in age (P value 0.006) and duration of disease (P value 0.014) in splenectomized children than non splenectomized children. Conclusion: The higher level of platlets in splenectomized children may be due to absence destruction of platelets by spleen.