Background: Polycystic ovary (PCO) is one of the most prevalent endocrinal disturbance among 
women during their child-bearing period. We aimed to highlight the serum levels of biochemical, 
endocrinal, and metabolic biomarkers of PCO in non-obese Egyptian women. Methods: This crosssectional study was conducted on 44 non-obese Egyptian women with PCO based on Rotterdam 
criteria in comparison with 44 healthy control women. The biomarkers levels were detected in the 
serum using fasting blood samples. Results: There was a significant difference between the two 
groups regarding the waist circumference and ratio between the waist and hip in the favor of the 
PCO group (P <0.001). Having the biochemical and endocrinal biomarkers, PCO group showed 
higher levels of glucose, insulin, triglycerides, cholesterol, low-density lipoprotein, the ratio 
between luteinizing-hormone and follicle-stimulating-hormone, vascular endothelial growth factor, 
17β-estradiol, and testosterone (P <0.001 for all). On the other hand, the PCO showed significant 
lower levels of progesterone, sex hormone-binding globulin, and high-density lipoprotein (P <0.001 
for all). There was no difference between the two groups regarding vitamin D and Kisspeptin (P = 
0.095 and 0.944, respectively). Conclusions: Many biomarkers were associated with the risk of 
PCO development among non-obese women.