Background: Urinary tract infections (UTIs) are among the commonest infections both in community 
and hospitals that showed emergence of multidrug resistance which is challenging UTI treatment. 
This study aimed to detect the prevalence of UTI, describe clinical presentations of UTI patients and 
define different bacterial uropathogens and antimicrobial susceptibility patterns of isolated 
Enterobacteriaceae species. Materials and Methods: From July 2016 to March 2017; 1353 urine 
specimens were collected from outpatients and inpatients presented to Minia university hospitals, 
Minia, Egypt. Demographic and clinical data of subjects were collected. Uropathogens were isolated 
on UTI chromogenic media and identified according to their phenotypic criteria, antimicrobial 
susceptibility was performed using disk diffusion method. Results: UTI prevalence was 52.1% which 
was more prevalent among married adult females from rural communities with 78.3% of subjects had 
one or more risk factors for UTI. Enterobacteriaceae prevalence, with E-coli being the most 
frequently isolated, was 62.4% among isolated uropathogens, 31.1% of them showed multidrug 
resistance (MDR). The highest rate of resistance was against sulfamethoxazole-trimethoprim (57.5%), 
Ceftriaxone (49.3%) and Amoxicillin clavulanic acid (36.1%) and highest susceptibility rate was 
found to Imipenem (100%). Conclusion: UTIs are common among Egyptian population and 
emergence of MDR Enterobacteriaceae strains among isolated uropathogens can lead to treatment 
failure. Proper antimicrobial polices and control of risk factors can lead to better management of 
UTIs.