Purpose: To assess the relationship of factor V leiden G1691A mutation as a risk factor for 
repeated pregnancy loss. The focus has been on factor V leiden G1691A mutation that may 
predispose women to microthrombosis during the stages of embryo implantation and 
placentation. Methods: A total of 70 women with recurrent pregnancy loss, mean age 
31.1±4.2 years, were involved in the study. As a control group, 70 women [mean age 
32.2±3.3 years with at least two live-born child and no history of abortion were included. We 
used real-time polymerase chain reaction (PCR) to determine the frequencies of factor V 
leiden G1691A genotype. Results: The frequency of heterozygotes for factor V leiden 
G1691A was significantly higher in women with repeated pregnancy loss compared to 
women without abortion (p = 0.0001). Conclusion: In summary we found an association of 
factor V leiden G1691A mutation with recurrent pregnancy loss .We recommend for factor V 
leiden G1691A screening in cases with repeated pregnancy loss so they can start 
anticoagulant therapy more earlier.