This study aimed to evaluate the protective effects of the ethanolic extract obtained from the seeds of 
phalaris canariensis, against nephro-toxicity induced by methotrexate (MTX). Toxicity was induced 
by a single dose of MTX (20 mg/kg) and ethanolic extract administered in three different doses (100, 
200 and 400 mg/kg) daily for 15 days. Urea and creatinine were measured in serum. In addition, 
malondialdehyde (MDA) and total nitrites, were measured in kidney tissues. At the same time, 
expression of kidney injury molecule-1 (KIM-1) and expression of BCL2 in kidney were measured. 
The ethanolic extract showed dose-dependent reduction in urea, creatinine, renal MDA, renal total 
nitrites and expression of KIM-1, which were increased with MTX administration, however, the 
extract dose-dependently, increased BCL2 expression that was reduced with MTX administration. in 
conclusion, this study suggests that the seed extracts from P. canariensis exhibit protective potentials 
against MTX-induced nephro-toxicity and can be considered a potential therapeutic agent in 
prevention of MTX-induced toxicity.