Objective: To detect the role of trefoil factor (TF2)(in both serum and urine) in different stage of 
CKD. Study design: A total of 40 patients with CKD and 20 apparently healthy volunteers (as 
control group) were eligible for the study. Patients group was subdivided into 3 subgroups {Mild 
(13), Moderate (13) and late group (14)}.Complete Blood Count(CBC),kidney function tests,Urine 
Protein /creatinine ratio ( P/C Ratio),Estimated glomerular filtration rate (eGFR), Serum and urine 
TF2 were done to all subjects. Results: Patients groups exhibited higher seruumTF2 concentrations 
(371.5 pg/ml) than control group (2 pg/ml). A significant increase in serum.TF2 level in group III 
when compared to group I, group II and group IV (p value= <0.001). Also, serum TF2 was 
significantly higher in group II when compared to group I and group IV (P value= <0.001). In 
addition there was significant increase in group I when compared to group IV (p value= <0.001).As 
regard urine TF it was higher in patients groups (460 pg/ml) than control group (2 pg/ml).A 
significant decrease in urine.TF2 level in group III when compared to group I, group II and group IV 
(p value= <0.001). Also, urine TF2 was significantly decrease in group II when compared to group I 
and group IV (p value= <0.001). But urine TF2 was significant increase in group I when compared to 
group IV (p value= <0.001). Conclusion: The data suggest the role of TF2 concentration (in urine 
and serum) to detect the stage of CKD. Serum TF2 concentrations increased progressively in later 
stages than early and moderated stage. Urine TF2 levels were significantly higher in early and mid 
CKD stages as compared to later stages.