Neonatal sepsis continues to be a global problem with significant morbidity and 
mortality. The diagnosis of neonatal sepsis is complicated by nonspecific clinical symptomatology, a 
high-false negative rate, and a delay in obtaining blood culture results. Subjects and Methods: The 
prospective cohort study was conducted in the Department of Clinical pathology and Neonatal 
Intensive Care Unit (NICU) at Minia University hospitals during the period from August 2014 to 
October 2015. Neonates were classified into two groups: group I: included 60 neonates with sepsis 
divided into 2 subgroup, 36 EOS (subgroup Ia) and 24 LOS (subgroup Ib), 27 female, 33 male, their 
ages ranged from 1 day to 27 days. group II: included 30 apparently healthy neonates 8 female, 22
male their ages ranged from 1 day to 24 days who served as a control group of matched age and sex. 
Results: The present study included ninty neonates, sixty with sepsis (group I) in addition into thirty 
apparent healthy neonates who served as a control group (group II) of matched age and sex.
Recommendation: Proadrenomedullin may be used as a routine investigation for early diagnosis of 
neonatal sepsis besides blood culture and procalcitonin. Further studies on larger population are 
recommended to investigate the prognostic value of proadrenomedullin.