Background: Urinary tract infections (UTI) during pregnancy issued by inappropriate laboratory
investigations situate women at high risk of several complications. Untreated and neglected 
asymptomatic UTI may lead to pyelonephritis, preterm birth, low birth weight, etc. Urine culture is
used to confirm the diagnosis of UTI. Objectives: This work aims to find the prevalence of 
asymptomatic UTI and to establish antimicrobial drug susceptibility of the pathogens resulting 
asymptomatic UTI in pregnant women attending at ante-natal care clinic at Minia Obstetrics 
University Hospitals. Methods: The urine samples were collected through mid-stream clean catch 
method from 90 pregnant women of age15-45 years visiting for ante-natal clinic. All the urine 
samples were subjected to biophysical, biochemical, microscopic, and microbiological analysis. The
prevalence of UTI in three trimesters and in different age groups was obtained together with
antimicrobial drug susceptibility tests. Results: On overall (38.8%) of urine samples revealed with
significant bacteriuria and considered as the asymptomatic UTI. On One way ANOVA test, there was 
significant (P<0.001) prevalence of asymptomatic UTI when compared among different trimesters, 
higher prevalence of asymptomatic UTI being in the third trimester (84%). Prevalence of culture 
positivity with respected age was maximum between the age group of 25-30 (41.4%). Escherichia coli
were the most implicated organism (52.8%) followed by Klebsiella pneumonae (18.6%). The gramnegative bacteria (E. coli and K. pneumonae) were more sensitive against Amikacin, Gentamycin, and
Nitrofurantointhan. Other drugs whereas; gram positive bacteria had high susceptibility with
Vancomycin. Conclusion: There was remarkable incidence of asymptomatic UTI among the pregnant
women in the study region; therefore, screening of urine of all pregnant women is very important and
should be included in the routine antenatal checkups to detect the symptomatic UTI to prevent the
maternal and obstetric complications in them.