A field experiment was conducted at Mallawi Agriculture Research Station Farm –(latitude of 27.73 ^o 28′ N and longitude of 30.83^o 95′ E) El-Minia Governorate, Egypt in two successive seasons of 2016/2017 and 2017/2018 to find out the influence of nitrogen fertilization and Titanium dioxide nanoparticles TiO₂ on yield and quality of three sugar beet varieties. Varieties exhibited significant differences in root length and sugar recovery in the two growing seasons, whereas the differences in TSS% were not significant. The differences among varieties in pol% and sugar yield were significant only in the second and the first growing season, respectively. Nitrogen fertilization had significant effects on root length, pol%, sugar recovery and sugar yield in both seasons, meanwhile it has a significant effect on TSS% only in the first season. TiO₂NP concentrations had significant effects on root length, pol%, sugar recovery and sugar yield in the two growing seasons, meanwhile it has insignificant effect on TSS% in both seasons. The second order interaction had insignificant effect on root length, TSS% and sugar yield in both seasons, meanwhile it has significant effects on pol% and sugar yield in the two growing seasons. Under conditions of the present work, it is recommend to fertilization Hercule or Kawemira sugar beet varieties by 60 or 80 kg N/fed. and 300 or 200 ppm TiO₂NP to produce the best quality as well as the highest sugar yield/fed.