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262083

BACTERIOPHAGES SPECIFIC TO A PHOSPHATE DISSOLVING BACTERIUM (BACILLUS MEGATERIUM) I- ISOLATION AND CHARACTERIZATION OF BACTERIOPHAGES

Article

Last updated: 05 Jan 2025

Subjects

-

Tags

Biotechnology (Agricultural Chemistry, Agricultural Microbiology and Genetics)

Abstract

Ten isolates of Bacillus megaterium bacteriophages were isolated from a soil sample collected from the Experimental Farm of Faculty of Agric. Minia University. The isolated phages produced single plaques circular in shape, clear in appearance and their diameters ranged from 1 to 3 mm. The isolated bacteriophages were tolerant to pH 4 upto 12. pH 8 was the optimal for the isolated phages. The thermal inactivation point of six phages (Nos. 1,3,4,7, 8 and 9) was 85°C. Whereas, phages Nos. 2,5, 6 and 10 displayed different thermal inactivation point (95°C). Therefore, the ten phages were classified to two groups. Group (A) contained phages Nos. 1,3,4,7, 8 and 9 and group (B) included phages Nos. 2,5, 6 and 10. Sensitivity of phage isolates to U.V. radiation was studied. Phages of group (A) lost their infectivities after exposure for 60 min to U.V., whereas, phages of group (B) inactivated after 40 min. The host range of phages of each group was the same. The electron micrographs of the phages indicated that phages of each group were identical in sizes and morphologies. Therefore, the phages of each group were belonging to one phage type. i.e. the phages of group (A) were one phage type and designated Bm1 and phages of group (B) were another type and designated Bm2.

DOI

10.21608/mjard.2022.262083

Keywords

Bacteriophages, Bacillus megaterium, phosphate dissolving bacterium

Volume

42

Article Issue

3

Related Issue

36806

Issue Date

2022-12-01

Receive Date

2022-09-29

Publish Date

2022-12-01

Page Start

195

Page End

206

Print ISSN

1110-0257

Online ISSN

2812-6505

Link

https://mjard.journals.ekb.eg/article_262083.html

Detail API

https://mjard.journals.ekb.eg/service?article_code=262083

Order

262,083

Type

Original Articles

Type Code

2,161

Publication Type

Journal

Publication Title

Minia Journal of Agricultural Research and Development

Publication Link

https://mjard.journals.ekb.eg/

MainTitle

BACTERIOPHAGES SPECIFIC TO A PHOSPHATE DISSOLVING BACTERIUM (BACILLUS MEGATERIUM) I- ISOLATION AND CHARACTERIZATION OF BACTERIOPHAGES

Details

Type

Article

Created At

23 Jan 2023