Although it had been demonstrated that honeybees have required some vitamins,there had been few attempts to quantify these requirements. Our results highlighted the effectiveness of Phenoloxidase (PO) and Antioxidant (AO) activity in honeybee workers and vitamin types added with sugar solution as early and vitamins important indicators. There was a clear improvement in the antioxidant system in bee colonies that were provided with sugary feeding with vitamins mixture compared to other vitamins individually (A, B, C and E) and control ones. This suggests that honey bee workers need artificial feeding with mixed vitamins during winter season possess mechanisms that reduce their oxidative stress. Together, ourresults suggest that vitamins in winter feeding for 14 weeks (from 1 December to 14 March) has the potential to improve colony development and health during overwintering, a period of high colony losses. The results shoued that vitamins diet had the potential to improve colony development and health during winter, the led to the absence of Eroupean foulbrood disease and reduction of varroa infection compared to the control.