The objective of this study was to estimate effect of three bread wheat varieties and seven mineral and bio-nitrogen fertilization on some agronomic traits and grain yield and its components. The experimental design was split–plot with three replications. Three bread wheat varieties (Giza 168, Sids 13 and Sids 4) were randomly allocated in the main plot. The subplots were seven fertilization treatments (bio N fertilizer, Mineral N fertilizer, 100%MN+bio, 80%MN+bio, 60%MN+bio, 40%MN+bio and 20%MN+bio) during the two successive growing winter season 2015/2016 and 2016/2017 at the Exp. Farm Fac. of Agric., Minia University., El-Minia, Egypt. Mean square of varieties, fertilization treatments and their interaction showed significant difference for most of the studied traits in the two growing seasons. Sids 4 and Sids 13 were recorded the highest grain yield 7.39 in 1^st season and 6.84 ton/ha in 2^nd season, respectively. Treatment 80%MN + Minia azoten bio fertilizer resulted in the highest grain yield per ha 7.67 ton and the highest harvest index 33.76 % in 2^nd season. This indicated that Minia azoten bio-fertilizer caused increase in grain yield/ha and harvest index with save 20 % from mineral nitrogen fertilization. Sids 13 with 80%MN+Minia azoten bio fertilizer recorded the highest grain yield per ha (10.36 ton) in 1^st season. Giza 168 with the same treatment gave the highest grain yield (8.78 ton/ha) in 2^nd season.