In order to investigate the effect of spraying Manfalouty pomegranate trees with chitosan at 0.0 (control), 50, 100, 200 and 400 ppm on yield (kg), fruit weight (g), number of fruit/tree as well as fruit physical and chemical properties of Manfalouty pomegranate (Punica granatium L), a field trial was conducted in two successive seasons (2018 and 2019) at a private farm located at El-Qusiya district (350 km southern Cairo city), Assiut Governorate. The obtained results confirmed that spraying Manfalouty pomegranate trees with chitosan at 50 ppm to 400 ppm significantly improved pomegranate fruits physical and chemical properties as well as yield (kg)/tree, fruit number/tree and fruit weight (g). This promotion was parallel with increasing chitosan concentration. However, non-significant differences were observed between the two highest concentrations used (200 and 400 ppm), during the two experimental seasons.