Eggplant production was limited by many plant pathogens all over the world. Root-knot nematodes (RKN) considered one of the most warning pathogens. In this study, the potential nematicidal effect of different spent mushroom (SM) doses i.e:10,20,30,50 and 100 g/pot were evaluated against Meloidogyne spp. infecting eggplant under greenhouse conditions. Results showed that all used SM doses significantly reduced nematode parameters compared to that contain nematode alone. Reduction in gall numbers reached 96% by adding SM at 100g/pot, followed by 91% at 50g/pot comparing with untreated control. Additionally, reduction of egg mass numbers and females / root system recorded 94.78 and 96.65 % respectively. Also, number of second stage juveniles/ 250g soil was affect by using the treatments. Moreover, plant growth parameters were significantly enhanced by most treatments in comparison with untreated control plants. Therefore, using of SP might be included in the integrated nematode management programs as a sustainable eco-friendly strategy.