Bean yellow mosaic virus (BYMV) was isolated from naturally infected bean
(phaseolus valgaris, L. cv. Karnk) and faba bean (Vicia faba, L cv. Balady) plants
were collected from different districts in menoufia governorate, Egypt; showing vein
chlorosis followed by green and/or yellow mosaic symptoms that may be due to virus
infection. Diseased samples were subjected to identification of isolated virus based
on symptomatology host range, serological tests (DAS- ELISA, Tissue blot
immunoassay TBIA and electron microscopy). Histological studies of bean infected
leaves by BYMV cleared that chloroplasts contained starch grain turned to spherical
in shape and with lost of their envelopes totally or partially. Chlorophyll a, b and
carotenoids as well as oxidative enzymes (peroxidase and polyphenol oxidase) were
determined in viral infected leaves. Molecular detection of BYMV using specific
primers was detected using RT-PCR; its products showed a single amplified
fragment of ~ 900 bp in electrophoresis analysis.