Experiment was carried out to evaluate the effective application methods of P. penetrans isolates from Egypt (Pp EGY) and Japan (Pp JAP) on root-knot nematode (RKN) Meloidogyne spp. control on eggplant under greenhouse conditions. Two application methods, spore suspension and powder of Pp EGY and Pp Jap used during the experiment. Results indicated that Pp EGY and Pp JAP showed a high reduction of root-knot nematode gall numbers compared to the control. Infected females of RKN with Pp EGY were markedly significant compared to Pp JAP. Plant growth was greatly influenced when both Pp EGY and Pp JAP were used. fresh shoot root and shoot dry weights were increased markedly compared to the control. In addition, the results indicate that P. penetrans offers a satisfactory and environmentally friendly solution for controlling root-knot nematodes.