Abstract: The current investigation was carried out to examine the possible potential protective effects of safflower against benzo [a] pirin -induced cancer liver. This study was conducted on thirty six male albino the rats divided into two main groups; the first main groups group (n= 6 rats) was fed on the basal diet And used as anegative control (–ve) normal rats. The rats of the second main group (n= 30 rats) fed basal diet and injected benzo[a] Pirin (10.08 mg/kg) in the previous cancer liver induction study and then divided into four subgroups (n=6 rats) as follows: group (2) fed basal diet without any treatment and used as a positive control (C+ve)Groups (3,4,5&6) fed on basal diet containing 20% pan breadfortified B_PCW, B_PFS1, B_PFS2, B_PFS3 & B_PFS4 for 28 days. Results indicated that, injected benzo [a] pirin cancer liver groups showed high significant increase in serum AST, ALT, ALP, total lipids, total cholesterol, triglycerides, LDL cholesterol, urea, creatinine, MDA and tumor necrosis factor TNF,α. While body weight gain, feed intake, feed efficiency ratio, total protein, HDL, SOD and total antioxidants were significantly decreased compared to control positive group. Treatment with pan bread fortified with safflower powder attenuated these adverse effects and ameliorated the evaluated biochemical parameters. The best result was pan bread for B_PFS at high level 40% , 30 % followed by 20% fortified with safflower powder diets compered to pan bread with 100% B_PCW. In conclusion, the results demonstrate that safflower has a potent protective effect against benzo [a] pirin -induced cancer liver injury in rats. The results also revealed that the effect of pan bread with safflower powder on the liver cancer may be attributed to its antioxidant and free radical scavenger.