In the present study, pumpkin seed oil was used as a nutritional source of different bioactive compounds.  Also, examined new insights on its roles that posed promising biological activities including organ functions manipulation, oxidant/antioxidant status improvement and obesity prevention will be in the scope of this study. Forty eight normal male albino rats (140±10g) were randomly divided into eight groups (6 rats per each) as follow:  group (1) fed on standard diet only and used as a negative control group,  group (2) fed on experimental diet contains 10% Supplier oil , group (3) fed on experimental diet contains 20% supplier oil, group (4) fed on experimental diet contains 15% supplier oil + 5% pumpkin oil, group (5) fed on experimental diet contains 10% supplier oil + 10 % pumpkin oil, group (6) fed on experimental diet contains  5% supplier oil + 15% pumpkin oil, group (7) fed on experimental diet contains 20% pumpkin oil and group (8) fed on experimental diet contains 10% pumpkin oil.