Purslane (Portulaca oleracea L.) in Arabic (Rejlah) is a member of Portulacaceae family and used in folk medicine as antidiabetic and hypotensive plant. The currently study was designed to assess hepatoprotective effects of aqueous purslane juice against CCl₄-induced hepatotoxicity in rats. Thirty five male albino rats divided into 5 groups, 7 rats each, were used. Group I served as normal group, groups II, III, IV and V were injected subcutaneously with CCL₄ (2 ml/kg) to induce liver toxicity. Group II served as positive group, groups III, IV and V were given orally aqueous purslane juice at doses of 5, 10 and 15 ml/kg of b. wt, respectively. Serum (lipid profile), kidney functions, (liver enzyme) activities; and liver MDA concentration and GSH, SOD and CAT activity were determined. Histo-pathological examination of the liver was performed to determine the extent of cells damage induced by CCl₄. Results showed that untreated hepatotoxicity rats have significant increase in serum TL, TG, TC, total and direct bilirubin, BUN and creatinine, GGT, AST, ALT, ALP; and liver MDA concentration, while decreased TP, albumin levels, liver activities of GSH, SOD and CAT, compared to normal rats. Aqueous purslane juice significantly improved all of the above parameters which were getting better with increasing the dose of juice. Histopathological assay showed multifocal hepatic cell necrosis, pericentral hepatic cell necrosis and focal area of mononuclear cells infiltration in positive rats and treated rats with 5 and 10 ml/kg b. wt of aqueous purslane juice, respectively. Congested central vein was showed in some livers and apparently normally central veins and hepatocytes in other livers of treated rats with 15 ml/kg b. wt of aqueous purslane juice. In conclusion, purslane juice has hepatoprotective effect against hepatotoxicity in rats. These results affirm that the traditional use of purslane plant for the protection of hepatotoxicity may be beneficial for patients with liver diseases and hence it is worth studying on humans.