Metabolic syndrome is defined as the co-occurrence of several cardiovascular risk factors, containing, insulin resistance, obesity, atherogenic dyslipidemia, and hypertension. Herpes simplex virus is an intracellular pathogen that can affect the skin of several parts of the body including the urogenital region, -mouth, eyes, and nervous system, which is life-threatening for children. The present study aimed to assess the percent of antibody of herpes simplex virus type 1 among children with metabolic syndrome (diabetes and obesity) in Primary schools in Ismailia city. Thus, a cross-sectional study was conducted on 46 children from age 6 to 10 years old in Ismailia city. Our method depend on dictation of herpes simplex virus type 1 antibodies (IgG) by commercial ELISA techniques. Serum triglyceride HD, L, LDL and fast blood sugar were measured by using colorimetric assay in accordance with a standardized method, they were analyzed through spectrophotometer Semi-Automatic clinical chemistry analyzer (Micro lab 300-ELITechGroup). In present study we found that a very high frequency of HSV-1 infections among the studied metabolic syndrome children with a percentage of 86% while the control group (children of the same gender and age) were 26% only. Upon the interpretation of the HSVs serological profiles, the past latent infection with HSV-1 (IgG was the most prevalent type of infection than HSV-1 recurrent infection (IgM) (0%), in all the HSV-1 positive cases (n= 46, 56%). Moreover, then there weakly positive correlation between IgG and triglycerides which is significant statistically (p< 0.05), while non-significant statistically correlation with HDL and LDL, Cholesterol, while ruling out the equivocal sample as HSV-1 IgM negative. The current study suggested that a very high incidence of HSV-1 antibodies among metabolic syndrome children in Ismailia city.