Effect of gold and silver nanoparticles on the detection of Ciprofloxacin in clinical wastewater by europium and terbium complexes. The synthetic fluoroquinolone Ciprofloxacin drug eliminate the bacteria via stopping protein and DNA formation by the interaction with enzymes responsible for reproductions. It is an established medication for many bone joints, cystitis, and respiratory and urinary system infections. It was considered as one of the final used treatment for such infections. Ciprofloxacin (CIP) was detected by two binary complexes Eu(III)-(PDCA)2 and Tb(III)-(PDCA)2 (PDCA= pyridine 2,6 dicarboxylic acid) in existence and nonexistence of different supports (silver and gold nanoparticles) using spectroscopic luminescence technique. The luminescent complexes were strongly quenched at 615 and 545 nm for europium and terbium complexes respectively, by ciprofloxacin at pH =7.4 in Tris-HCl buffer solution. The detection limits of CIP were 1.76µM, 1.49 µM, and 1.06 µM using Eu(III)-(PDCA)2, Eu(III)-(PDCA)2-AuNPs and Eu(III)-(PDCA)2-AgNPs, respectively. While in the case of Tb(III)-(PDCA)2, Tb(III)-(PDCA)2- Au NPs, and Tb(III)-(PDCA)2-AgNPs, the detection limits of CIP were 2.05 µM, 1.77 µM, and 1.46 µM, respectively. This method of detection showed a good retrieval for CIP identification in hospital wastewater.