Pollination is a critical process in date palm production lines that affects yield and fruit quality. This study aimed to evaluate the effect of sprayed pollen grain powder on yield and fruit quality of Madjool, Segae and Khodry date cultivars. This study was conducted in Dakhla Oasis, New Valley Governorate, Egypt during the 2019 and 2020 seasons. Results showed that the most beneficial treatment in this concern is spraying female spathes with 0.5 to 1 g pollen grains plus 10% sugar/L water which gave economical yield with good fruit quality. Moreover, it is distinguished to increase the pollination efficiency and save time, effort, labour and cost likewise more practical because it plays a promising in pollination technique in the future.