Background: End stage of kidney disease (ESKD) the last stage of renal failure and can develop from untreated chronic kidney disease. The study aimed to evaluate the effect of nursing guidelines for Buttonhole cannulation technique of arteriovenous on reducing its complications among hemodialysis patients. Research design: This research employed a quasi-experimental methodology. Setting: The study was conducted in Minia University at Nephrology and Urology Hospital at Hemodialysis Units. Subjects of the study: A purposive sample composed of sixty patients going for hemodialysis, study participants was classified equally for both study and control groups (thirteen for each one).Tools of data collection In order to conduct the current study, four data gathering methods were employed, including; A Structured interviewing questionnaire sheet, Pain assessment scale, Observational Checklist for Manifestation of infection and hematoma and assessment sheet for Laboratory investigation of kidney function. Results: the findings of the study indicated that 13.3% among the study group & 36.7% of the control group were had hematoma at 1st week of HD after the application of study guidelines, while 3.3% & 86.7% among both   groups were had hematoma at the fourth week of HD after the application of study guidelines  respectively. Conclusion: Buttonhole study guidelines was had positive impact on hemodialysis complications (pain, infection, hematoma, vital signs) for study group in first and fourth week in hemodialysis sessions in- comparison with control group. Recommendations: Increase awareness through nursing staff of hemodialysis patients and caregivers regard self-care related to vascular access and encourage patient participation in health education programs about evidence-based practice regard hemodialysis care at home.