Background: Coronavirus disease (COVID-19) is an illness caused by a novel coronavirus, now called Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). Aim: to assess knowledge and practice toward coronavirus disease among patients with selected chronic diseases. Design: A descriptive cross-sectional design was utilized. Setting: The current study was conducted at the medical, diabetic, chest, and cardiac out-patient clinics, at Minia University Hospital and Cardio-Thoracic Hospital. Sample: A purposeful sampling consisted of 331 patients with chronic diseases (hypertension, diabetes mellitus, heart disease, and chronic lung disease) Collection of data: Data were collected using two tools Second tool( 1^st tool): It was included three parts: Part 1: Socio-demographic characteristics, part 2: Lifestyledata and  Part 3: Knowledge of chronic disease patients toward coronavirus disease: 2^nd tool: Practice of chronic disease patients toward coronavirus disease: Results: The main finding of the study 83.4.% of the studied sample had age ranged from 30 ≤ 49 years and 50 ≤ 69 years respectively with mean of (50.05 ± 9.90) and 63.1% of them were male. The main finding of the study 56.2% of the studied sample had poor knowledge and 73.1% of them had poor practice toward coronavirus disease. Conclusion: This study showed that poor knowledge and practice were found among the studied sample. There was a positive strong correlation between the total score of knowledge and the total score of practices of the studied sample toward Coronavirus disease. Recommendations: Educational workshops and periodical programs should be conducted for all people to increase their knowledge, attitude, and practice regarding the coronavirus disease.