Background: Multiple sclerosis (MS) is the most common autoimmune disease affecting the central nervous system (CNS). It is a demyelinating disease of the central nervous system, which leads to impaired cognitive, motor and or sensory functions. Aim: The study aimed to assess health related quality of life among patients with multiple sclerosis at Minia University Hospital. Design: Descriptive research design.Sample: A convenience sample consists of 60 patients with multiple sclerosis. Settings: The present study was conducted at out patient's neuropsychiatric clinic at Minia university hospital. Tools: Data were collected by two tools; the first tool: Personal Data; the second tool: Multiple Sclerosis Quality of Life (MSQOL) structured interviewing questionnaire. Results: The current study shows that the mean age was 33 ± 7.7 years and two-thirds of them were females, also, 50% of sample has fair quality of life. There are highly statistically significant difference between overall quality scores and physical health, role limitations due to physical problems, role limitations due to emotional problems, pain, emotional well-being, energy, social function, cognitive function and health distress. Conclusion: The current study concluded that there was significant impairment in quality of life for multiple sclerosis patients, in addition that multiple sclerosis patients suffer from impairment in their quality of life among all domains. Recommendations: Perform a continuous health assessment and observation to the multiple sclerosis patients (health status, emotional status, skin integrity, pain sensation, and balance and movement ability).