Background: Central venous catheter is required in multiply injured patients either in the initial resuscitation phase or during an intensive care unit stay. There are potential complications associated with central line access as infection. Nurses play a crucial role in preventing this infection. Aim of the study: To evaluate the effect of nursing guidelines on reducing central line related infection among traumatic patients. Design: Aquasi-experimental research design.Sample: A purposive sample of 60 newly admitted adult patients with central venous catheter divided equally into two groups (study and control). Setting: The current study was conducted in traumatic intensive care unit of Qena university hospital, Qena governorate, Egypt. Tools: Two tools structure interview questionnaireand central line related infection assessment sheet. Results: There were highly statistically significant differences between the study and control groups regarding central line related infection with (p < 0.001). Conclusion: The application of nursing guidelines was effective in reducing central line related infection among traumatic patients. Recommendations: Nursing guidelines for preventing central line-related infection should be educated for nurses of intensive care units in Qena university hospital.