Aim: The study aimed to assess effective of educational instruction of nurse's knowledge regarding meningitis and universal precaution measures at selected department at Minia Fever Hospital. Study design: A descriptive cross-sectional research design was utilized in this study. Subject: A convenient sample of all nurses at two department of Fever Hospital in Minia city (kidney department and pediatric department), that include 50 nurses who devided into 14 nurses had abachlor degree, 10 nurses had deploma in nursing and 26 nurses had Technical institute. Setting: This study was carried out at Fever Hospital in Minia city. Tools: Three tools were utilized in this study, Results The most of study subjects have unsatisfactory level of knowledge about meningitis with mean scores 9.3±2.7 and the most of study subjects have good knowledge about universal precautions regarding meningitis with mean scores 24.4±6.8. Conclusion: the most of study subjects have unsatisfactory level of knowledge about meningitis and less than fifth have satisfactory level of knowledge about meningitis and the most of study subjects have good knowledge about universal precautions regarding meningitis. Recommendations: Encourage the nurses to participate in training courses held by specialists in meningitis to update their knowledge and increase nurse's knowledge and updated in pediatric unit in hospital to the current researches and guidelines by training course.