Aim: The study aimed to evaluate the effect of health educational program on mothers' knowledge and practices regarding care of children with trachoma. Study design: quasi-experimental design (pretest-posttest) used to conduct this study. Sample: The study sample includes 60 mothers caring for children with trachoma. Setting: This study was conducted at outpatient Ophthalmological clinics of Minia University hospital and ophthalmology hospital in Minia City .Tools: Three tools were utilized in this study; the first tool was an interviewing structured questionnaire sheet. The second tool: Mothers' knowledge regarding trachoma, and the third tool: self-reported practices scale. Results the minority (5%) of the studied mothers had high level of knowledge pre-educational program that increased to most (91.7%) of them immediately after the end of the educational program and a majority (88.3%) of them after one month of educational program with statistically significant differences. More than one third (41.7%) of them had good self-reported practices level pre-educational program increased to most (91.7%) of them immediately after the end of the educational program and more than majority (88.3%) of them after one month of an educational program with a highly statistically significant difference. Also, there was a strong positive association between mothers' knowledge and their reported practices regarding trachoma at pre-educational program. Conclusion: The teaching program is effective in improving the knowledge and practices of mothers. It found that levels of knowledge and practices of mothers posttest were significantly higher compared to pretest.