Background: clinical audit is a complementary part of the standards it includes a checklist and action plan. The checklist can be used to test or audit standards. The action plan is a conclusive part of the audit. Iron deficiency anemia during pregnancy is one of the most significant health problems in the world. Impact of iron deficiency anemia on the pregnant women its outcomes that it has many serious consequences on increase the risk of fetal growth retardation, premature delivery. Aim of the study: To assess clinical audit which used for the pregnant woman with iron deficiency anemia. Research design: descriptive design was carried out in this study. Setting: All Maternal and Child Health centers in Qena City and Minia City which were four centers in Minia and six centers in Qena. Sample: convenient sample of 500 records of pregnant women.  Tool of data collection: Tool 1: questionnaire sheet includes the socio-demographic include personal and obstetrical history. Tool 2: Standard of care for the pregnant with iron deficiency anemia adopted from (WHO) source. Results: we found that 76% in Qena city versus 82%in Minia city had mild anemia, while 24% in Qena city versus17.6% had moderate anemia. Absence of woman with severe and very severe anemia .distribution of Mild and Moderate anemia was higher in urban. More than 75% of standard points were applicable in the two governorates. Conclusion: The present study concluded that the nurses of Maternal and Child Health centers didn't apply all points of WHO audit. Recommendations: Every healthcare facility should develop and implement an Annual Clinical Audit Forward Plan as part of its annual planning and delivery cycle for clinical audit activities and the facility's safety and quality governance framework. Health care providers  need further training to apply clinical audit  completely .Health care records needed updated to conclude all point of WHO clinical audit  related to absence of some of care services to pregnant woman.