Background: Evidence-based practice (EBP) is a systematic approach to improving the quality of nursing practice.Aim: The current study aims to investigate the effect of educational program regarding evidence based practice on beliefs and implementation among Clinical instructors. Design: A quasi-experimental research was utilized. Setting: The study was carried out at Faculty of Nursing, Minia University. Subjects: convenient sample of all available (78) clinical instructors who were working in a six Departments in Faculty of Nursing at Minia University at the time of conducting the study. Tools: Two tools were used; the Evidence-Based Practice Beliefs (EBPB) Scale, and the EBP implementation (EBPI) scale. Results: showed that total mean scores of clinical instructors' evidence-based practice implementation was increased post program than pre-program and follow up session was increased than pre and post program and total mean scores of clinical instructors' evidence-based practice beliefs was increased post program than pre-program and follow up session was increased than pre and post program with highly statistically significance differences which P – value .0001 respectively. Conclusion:  Findings of the research demonstrated that education can affect the clinical instructor's belief and implementation to use EBP. Recommendations: Repetition of the educational program in other nursing Faculties needs to be done on the use and implementation of EBP.