Background: Students academic incivility is a serious and growing concern in higher education and specifically in nursing education. Incivility is increasing among nursing students and it is one of the problems affecting nursing education in different countries. Aim: To assess perception and incidence of students academic incivility at Faculty of Nursing. Research design: The study was conducted using descriptive research design. Setting: The study was carried out at Faculty of Nursing, Minia University. Subjects: The study subjects consisted of30% from total number of nursing students enrolled at 2nd, 3rd, and 4th academic years during the academic year 2018-2019 (N=400), and 50 % from total number of faculty staff members and their assistants (N=61). Tool: Incivility in nursing education survey (INE) was used for data collection. Results: Revealed that, staff members had the highest level of perception regarding student academic incivility behaviors with (88.5%) followed by students with (73.8%). Also, the highest percent regarding incidence of students disruptive behaviors was for moderate level of occurrence from staff members view with (75.4%) followed by students view with (49.8%). Moreover, the highest percent regarding incidence of student threatening behaviors was for low level of occurrence from students view with (71.8%) followed by staff members view with (59%). Conclusion: There is statistically significant difference between students and staff members view regarding perception of students' academic incivility behaviors and its incidence. Recommendation: Creating and implementing new student orientation program, which considered a prim opportunity to prepare students for their undergraduate experiences by providing valuable resources and information. And staff members should be role model in civil discussion, communicates effectively with the students in the class, and focus on creating respectful relationship with them and appreciating their effort and perspectives.