Background: Hemodialysis places children at high risk for infection because of patient comorbidities and numerous human, environmental, and procedural factors. The aim of this study was to evaluate the effect of the educational program on the nurse's knowledge and their practices about infection control measures in hemodialysis units. Research design: Pre /post quasi experimental design was used in this study.  Sample: A convenient sample of all nurses in hemodialysis units, Setting: the study was conducted in pediatric hemodialysis units at Minia University and Minia General Hospitals. Tools for Data Collection: A structured interview questionnaire sheet in Arabic language was used for the studied nurses as pre/posttest. It composed of the following parts: Part I: Demographic data related to the nurses' age, sex, qualification, years of experience and previous attending courses in infection control. Part II: Nurses, knowledge related to infection control in pediatric hemodialysis unites Part III: An observational checklist that was adopted from Control Diseases Center. (National Center for emerging and zoonotic infectious diseases division of health quality promotion). Results: the finding of the current study revealed that there were statistical significant differences between nurses' knowledge and practices at pre and post- program. There were significance correlations between nurses' knowledge, residence and occupation respectively at pre- program.  Conclusion the study concluded that there were statistical significant differences between nurses' knowledge and practices at pre and post- program. Recommendation the study recommended  that the developed  program  should  be  applied  and  repeat again  every  6  months  as well as provision   of   continuing   education programs  on  regular  basis  is  suggested  in  order  to refresh  and  update  nurse's  knowledge.