Background: Conflict is a fact of human lives and if they are able to understand it and its impact on work effectiveness, they can make conflict useful and use them to achieve better results Aim of the current study is to identify organizational conflict causes and resolution style among first line managers and hospital administration. Design: A descriptive research design was utilized in the current study. Subjects of this study compromised convenient sample of all available nurse) first line managers who working at hospitals during the period of data collection (no 156).  Setting: the study was carried out at Minia University Hospitals. Tools: two tools were used to collect data of the study; Organizational Conflict Causes Questionnaire and Organizational Conflict Resolution Styles Scale. Results: The study results revealed that three quarter of hospital administrative and first line manager agreed about causes of conflict. The most conflict styles used by hospital administrative were collaborating, accommodating, and competing while, first line manager preferred compromising, avoiding, and competing styles, with statistically significant difference. Conclusion: this study concluded that, all participants agreed about causes of conflict; as well hospital administrative preferred collaborative style and first line manager preferred compromising style. Recommendations: Establishment of training courses for all nursing educators to improve their skill for conflict resolution.