Background: The workers are exposed to workplace hazards arising from various activities involved in sugar production. Aim: study aims to assess effect of health education program on knowledge and practice of workers regarding occupational health hazards at sugar factory. Design: Quasi-experimental design was utilized for this study with pre/posttest. Sample: A purposive sample with number of 130 workers according to specific criteria. Setting: The present study was carried out in Minia sugar factory in Abu Korkas district, Tools: Data were collected using three tools; the first tool included questions related to demographic characteristics, health history, health habit and occupational health services. The second tool is a questionnaire regarding knowledge of workers about occupational health hazards and the third tool is a self-reported practice questionnaire about using workers personal protective equipment and most commonly first aid in the factory. Results: the mean age was 44.7 ± 8.7, 37.7% years. All workers of the study sample had poor knowledge about occupational health hazards and unsatisfactory self-reported practice regarding personal protective equipment and first aids. Conclusion: post implementation of health education program the workers knowledge and self-reported practice improved. Recommendation: on-site training programs for workers have to be implemented to improve their working skills. Training programs should include awareness about safety measures and orientation regarding work hazards and how to avoid work related injuries as well as first aid measures.