Background: Accidents are a major cause of morbidity and mortality in children. Along with cardiovascular diseases and cancers, accidents currently form the 3rd leading causes of morbidity and mortality in developed and developing countries. Aim: The study aimed to evaluate the effect of accident prevention program on nursery school teachers' knowledge and practices. Research design:A pre/post quasi-experimental research design was used in this study. Sample: A convenient sample of 60 nursery school teachers, 30 from private and 30 from governmental nursery   schools. Setting: The selected nursery schools represented all geographic areas of Minia city. Tools: pre-designed questionnaire sheet was used to collect data from teachers which include part 1: Demographic data about teachers as age, marital status, qualifications, years of experience and previous training courses. Part 2 Teachers' knowledge about accident prevention and first aid. An observational checklist was used for   first aid measures. Results: The study results revealed that the majority of the studied teachers were married and more than two fifth of them had >30 years old with a mean of age 33.32 ± 7.478.years. The total mean scores of both knowledge and practice were increased after implementation of the program with statistical significant differences at (p-value 0.0001) between   pretest, immediate posttest and posttest after 3 months. Conclusion: The current study concluded that there was an increase in the mean scores of teachers' knowledge and practices after implementation of the program about accident prevention and first aid. Recommendation: The study recommended a wide coverage implementation of this research about accident prevention and first aid for the nursery school teachers all over Minia City nursery schools.