Background: Patient safety is the cornerstone of high-quality healthcare. Hemodialysis units are complex organizations that involve providers from multiple disciplines. Nursing interventions have a great impact on reducing the risk for complications of hemodialysis patients. Aim: develop, implement and evaluate patients' safety guidelines for nurses at hemodialysis units in Ministry of Health Hospitals at Beni-Suef Governorate.. Research design: -Methodological and  Quazi experimental was used in this study. Setting: This study was conducted in Ministry of Health Hospitals at Beni-Suef Governorate. All  staff nurses in hemodialysis units were included the study sample study, it composed of (45) nurses. Tools: two tools were used in the study A) Observation checklist and B) Patient safety guidelines. Results the total mean scores of nursing performance was 178.06+49 before implementing guidelines program which it became the highest total mean scores  was 280.02+35.5 after the implementation of guidelines for hemodialysis units. Conclusions: The developed  patient safety guidelines for nurses in hemodialysis units had a positive effect on nurses' performance in ministry of Health and Population's Hospitals in Beni-Suef Governorate.Recommendation Increase and well-organize in-service training program on hemodialysis units including the newly developed standard patient safety guidelines, Developed program and its booklet should be applied in all similar settings in Beni-Sueif as a step for wider application in other governorates .