Background: Nursing and medicine are two professions, which have many roles that interact with their responsibilities. Collaboration has been promoting as one of the best approaches for healthcare systems to adopt when trying to progress the outcomes Aim: assess the perception of nurses and physicians about the importance of collaboration in the work. Methods: A descriptive research design was used. Setting: The study was conducted at Beni-Suef University Hospital in four critical care units ICU,CCU,PCU,NSCU and medical, surgical department. subjects: A convenience sample was used. One hundred of nurses and fifty of physicians working at Beni-Suef University Hospital were included in the current study. Tool: included two parts part one personal characteristics questionnaire and part two collaboration perception scales for physicians and nurses. Results: the current study revealed that all of studied physicians had suboptimal perceptions regarding nurse/physician collaboration, and more than three quarters of nurses had suboptimal perceptions regarding nurse/physician collaboration. There was a highly significant relation between the mean score of collaboration perception scale for physicians and collaboration perception scale for nurses at p ≤ 0.01. Conclusion: Most nurses and all physicians have suboptimal perceptions regarding nurse/physician collaboration. Moreover, the mean score of collaboration perception scale for nurses and physicians has a significant relationship with gender, years of experience and all working place except for surgical department. Recommendations: encouraging inter-professional collaboration in healthcare institutions by managers and administration. Establishing a base for inter-professional collaboration between all healthcare team members in nursing and medical schools. Clearing job description for all categories of nurses and physicians.