Background: Family planning (F.P) is an important strategy in promoting maternal and child health. Contraceptive use is a key factor in preventing unwanted pregnancies, reducing maternal and child mortality, and improving the lives of women and their families. A recent study estimated that contraceptive use could avert more than two-fifths of maternal deaths. Aim of this study: was to assess the effect of implementing guideline instruction on awareness of newly married women regarding family planning methods Research question: what is the effect of implementing guideline instruction on awareness of newly married women regarding family planning methods?. Research design: A Descriptive cross sectional research design has been utilized in the present study. (Subjects and methods) Sample: Three hundred participants were included in this study. Tools: a structured interviewing questionnaire sheet developed by the researcher. Pretest was done to evaluate participant's knowledge regarding contraceptives then the guideline and health education for participants, Post evaluation was done to test improvement of their awareness after 3 months of intervention. Results: showed that level of knowledge regarding contraceptives among newly married women was poor in the pre test while after implementing the guideline and health education a great improvement in their level of knowledge was observed with high statistical significant difference (P≤ 0.001). Conclusion: health education and implemented guideline were effective in increasing the knowledge of women regarding family planning. Recommendations: Efforts should be made to strengthen the media for providing accurate knowledge about F.P programs, premarital family planning counseling for male and females, and increase quality of F.P services staff through training program and updating information.