Obesity is a primary reason for morbidity and mortality. The current study aimed to evaluate the anti-obesity and antioxidant effect of Moringa peregrina (MP) seeds oil in obesity-induced rats. Forty male Sprague Dawley rats were divided into four equal groups of 10 rats each. Group C (control); group OB rats were fed a high-fat diet (HFD). Group MPI: rats treated with HFD+MP seeds oil (250 mg/kg b.wt./day, orally) and group MPII. rats treated with HFD + MP seeds oil (500 mg/kg b.wt./day, orally). Blood samples were collected by the end of the experiment for determination of serum total cholesterol (TC), Triacylglycerols (TAG), high-density lipoprotein (HDL), and low-density lipoprotein (LDL), and liver functions (ALT, AST, ALP). Moreover, malondialdehyde level (MDA), superoxide dismutase and catalase activity were measured in both plasma and liver. The results showed that TC, TAG, LDL, ALP, AST, ALT and MDA were significantly higher in obese rats than in the control group. On the other hand, administration of MP seeds oil to HFD-fed rats improved oxidative stress and lipid metabolism abnormalities by ameliorating TC, TAG and liver enzyme activities. These results suggest that MP seeds oil effectively enhances obesity-associated parameters and decreases its related complications.