Natural products have succeeded in reducing toxicity. Ottelione is a natural product that has a powerful tubulin polymerization inhibitory effect and cytotoxicity in tumor cell lines. This study was planned to prove its therapeutic effect against hepatotoxicity induced by carbon tetrachloride. 55 mice were divided to six groups (n=11): 1st group as the negative control group (normal group) injected (i.p.) with olive oil, The2nd Group was treated (i.p.) with DMSO, 3rd group was treated with Ottelione injected(i.p.), 4th group (G4) was (positive control) injected by CCl4 to induce liver toxicity, and 5th group mice received CCL4 in olive oil. (i.p.) 3 times/week for 6 weeks, followed by Ottelione injected (i.p.) for 15 days (daily). The positive control group showed an increase in Gamma-glutamyl transferase and significant changes in hematological parameters when compared with a negative control group. Treating the mice with Ottelione showed an enhancement in Gamma-glutamyl transferase and in hematological parameters when compared with the positive CCl4 control group. Ottelione succeeded in reducing hepatotoxicity in mice induced by CCL4 and it could restore the liver to normal