Seven sites were surveyed for collecting samples of C4 and C3 plants of Poaceae family from aquatic and terrestrial habitats. C4 plants include Saccharum sp., Saccharum spontaneum, Echinochloa stagnina, Echinochloa colona and Echinochloa crusgalli. C3 plants include Phragmite australis and Leersia hexandra. Morphological description and anatomical characteristics of culm and leaf were studied in the two habitat. Growth rate and anatomical features of studied species affected by altering habitat from aquatic to terrestrial habitat. Morphological and anatomical characters were recorded and the data were analyzed using Past Software V. Cluster analysis was done depending on morphological characters and anatomical characters separately. The   relationship   between   the   studied   species depending  on  morphological  characters  different from  that  based  on  the  anatomical  characters. Also, the relationship between studied C3 and C4 species affected by altering the habitat from aquatic to terrestrial one.