In this study, the concentrations of heavy metals were measured in the sediment of the Eastern Alexandria Harbour collected twice during 2016. The range and average concentrations measured in ppm were (ND–2775.93) (1592.59) for Fe and (13.90 - 235.73) (71.36) for Zn, (ND – 8.77) (3.93) for Ni and (6.35 - 42.99) (25.04) for Cr. For clarity, Enrichment factor index (EF) was used to determine the level of sediment contamination. The pollution load index (PLI) calculated for all the sediment samples that fall in the second terminology (1(ERM) values. All the samples fall in the range below (ERL) for both of Ni and Cr.  And most of the samples fall in the range below (ERL) for Zn in (11) sample. While,  sample (1) only fall in the range between(ERL and ERM) for Zn.The bottom sediments serve as a reservoir for heavy metals and therefore deserve special consideration in the planning and design of aquatic pollution research studies.